data from solutions of
Blue light receptor YtvA, dark/inactive state
PBS + 5 mM DTT, pH 7.4
were collected on the
EMBL X33 camera
at the DORIS III storage ring
using a Pilatus 1M-W detector
at a sample-detector distance of 2.7 m and
at a wavelength of λ = 0.15 nm
(l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 1.7 and 11 mg/ml were measured
30 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.