data from solutions of
Blue light receptor YtvA, dark/inactive state
PBS + 5 mM DTT, pH 7.4
were collected on the
on the storage ring DORIS III
Using a Pilatus 1M-W detector
at a sample-detector distance of 2.7 m and
at a wavelength of λ = 0.15 nm
(s = 4π sin θ/λ, where 2θ is the scattering angle).
Solute concentrations ranging between 1.7 and 11 mg/ml were measured
30 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted
and the different curves were scaled for protein concentration.
The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.