Structural basis of apoptosis inhibition by the fowlpox virus protein FPV039.

Anasir MI, Caria S, Skinner MA, Kvansakul M, J Biol Chem 292(22):9010-9021 (2017) Europe PMC

SASDC63 – Fowlpox Virus FPV039 antiapoptotic Bcl-2 viral protein in complex with BaK BH3 peptide (FPV039:BAK)

Bcl-2-like protein FPV039
Uncharacterized protein (BAK1)
MWexperimental 18 kDa
MWexpected 20 kDa
log I(s) 1.04×10-1 1.04×10-2 1.04×10-3 1.04×10-4
Bcl-2-like protein FPV039 Uncharacterized protein (BAK1) small angle scattering data  s, nm-1
ln I(s)
Bcl-2-like protein FPV039 Uncharacterized protein (BAK1) Guinier plot ln 1.04×10-1 Rg: 2.0 nm 0 (2.0 nm)-2 s2
(sRg)2I(s)/I(0)
Bcl-2-like protein FPV039 Uncharacterized protein (BAK1) Kratky plot 1.104 0 √3 sRg

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Fowlpox Virus FPV039 antiapoptotic Bcl-2 viral protein in complex with BaK BH3 peptide in 20 mM trisodium citrate, 200 mM NaCl, pH 6.0 were collected on the SAXS/WAXS beam line at the Australian Synchrotron (Melbourne, Australia) using a Pilatus 1M detector at a sample-detector distance of 1.6 m (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Solute concentrations ranging between 0.31 to 7.35 mg/ml were measured. 30 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected were obtained from a single concentration scattering curve (7.35 mg/ml scattering data).

Wavelength = UNKNOWN

Bcl-2-like protein FPV039 (FPV039)
Mol. type   Protein
Organism   Fowlpox virus
Olig. state   Monomer
Mon. MW   16.8 kDa
 
UniProt   Q9J5G4 (1-143)
Sequence   FASTA
 
Uncharacterized protein (BAK1) (Bak)
Mol. type   Protein
Organism   Gallus gallus
Olig. state   Monomer
Mon. MW   2.8 kDa
 
UniProt   Q5F404 (72-97)
Sequence   FASTA