| MWexperimental | 25 | kDa |
| MWexpected | 27 | kDa |
| VPorod | 41 | nm3 |
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log I(s)
1.19×103
1.19×102
1.19×101
1.19×100
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s, nm-1
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Small angle X-ray scattering as a complementary tool for high-throughput structural studies.
Grant TD, Luft JR, Wolfley JR, Tsuruta H, Martel A, Montelione GT, Snell EH, Biopolymers 95(8):517-30 (2011) Europe PMCSASDC96 – N-terminal domain of Diguanylate cyclase with PAS/PAC sensor (Maqu_2914) from Marinobacter aquaeolei, Northeast Structural Genomics Consortium Target MqR66C
Diguanylate cyclase with PAS/PAC sensor|
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Fits and models
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Synchrotron SAXS
data from solutions of
N-terminal domain of Diguanylate cyclase with PAS/PAC sensor (Maqu_2914) from Marinobacter aquaeolei, Northeast Structural Genomics Consortium Target MqR66C
in
5 mM DTT 100 mM NaCl 10 mM Tris-HCl 0.02 % NaN3, pH 7.5
were collected
on the
BL4-2 beam line
at the Stanford Synchrotron Radiation Lightsource (SSRL) storage ring
(Menlo Park, CA, USA)
using a Rayonix MX225-HE detector
at a sample-detector distance of 1.5 m and
at a wavelength of λ = 0.13 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 2.6 and 7.1 mg/ml were measured
at 20°C.
20 successive
1 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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