WAXS benchmark on standard proteins

Maxim Petoukhov.

SASDCD8 – Horse spleen apoferritin (WAXS)

Horse spleen apoferritin
MWexperimental 540 kDa
MWexpected 476 kDa
log I(s) 5.11×104 5.11×103 5.11×102 5.11×101
Horse spleen apoferritin small angle scattering data  s, nm-1
ln I(s)
Horse spleen apoferritin Guinier plot ln 5.12×104 Rg: 6.3 nm 0 (6.3 nm)-2 s2
Horse spleen apoferritin Kratky plot 1.104 0 3 sRg

Data validation

Fits and models

log I(s)
 s, nm-1
Horse spleen apoferritin PDB (PROTEIN DATA BANK) model

Synchrotron SAXS data from solutions of Horse spleen apoferritin in tbs, pH 7.5 were collected on the EMBL-P12 beam line at the PETRA III storage ring (Hamburg, Germany), using a Pilatus 2M detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.062 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). A solute concentration of 15.8 mg/ml was measured at 20°C. 40 successive 0.095 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the data scaled for protein concentration.

Tags: benchmark
Horse spleen apoferritin (Apoferritin)
Mol. type   Protein
Organism   Equus caballus
Olig. state   24-mer
Mon. MW   19.8 kDa
UniProt   P02791
Sequence   FASTA