data from solutions of
HIT family hydrolase protein from Vibrio fischeri. Northeast Structural Genomics Consortium target id VfR176
5 mM DTT 100 mM NaCl 10 mM Tris-HCl 0.02 % NaN3, pH 7.5
were collected on the
on the storage ring Stanford Synchrotron Radiation Lightsource (SSRL)
(Stanford, CA, USA).
Using a Rayonix MX225-HE detector
at a sample-detector distance of 1.5 m and
at a wavelength of λ = 0.13 nm
(s = 4π sin θ/λ, where 2θ is the scattering angle).
Solute concentrations ranging between 2.1 and 6.3 mg/ml were measured
1 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted
and the different curves were scaled for protein concentration.