Characterizing protein dynamics with integrative use of bulk and single-molecule techniques.

Liu Z, Gong Z, Cao Y, Ding Y, Dong MQ, Lu Y, Zhang WP, Tang C, Biochemistry 2017 Sep 25; PubMed

SASDCG7 – Lys63-linked diubiquitin at pH7.4

MWexperimental 13 kDa
MWexpected 17 kDa
VPorod 22 nm3
log I(s) 6.32×101 6.32×100 6.32×10-1 6.32×10-2
Polyubiquitin-C small angle scattering data  s, nm-1
ln I(s)
Polyubiquitin-C Guinier plot ln 6.33×101 Rg: 2 nm 0 (2 nm)-2 s2
Polyubiquitin-C Kratky plot 1.104 0 3 sRg
Polyubiquitin-C pair distance distribution function Rg: 2.1 nm 0 Dmax: 7 nm

Experimental data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Lys63-linked diubiquitin in 20mM HEPES, 150mM NaCl, pH 7.4 were collected on the BL19U2 beam line at the Shanghai Synchrotron Radiation Facilitystorage ring (SSRF: Shanghai, China) using a Pilatus 1M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsin θ/λ and 2θ is the scattering angle). One solute concentration of 2.60 mg/ml (150 µM) was measured at 25°C. 20 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Polyubiquitin-C (ubiquitin)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   8.6 kDa
UniProt   P0CG48 (305-379)
Sequence   FASTA