WAXS benchmark on standard proteins

Maxim Petoukhov.

SASDCG8 – Bovine carbonic anhydrase (WAXS)

Carbonic anhydrase 2
MWexperimental 26 kDa
MWexpected 29 kDa
log I(s) 4.05×103 4.05×102 4.05×101 4.05×100
Carbonic anhydrase 2 small angle scattering data  s, nm-1
ln I(s)
Carbonic anhydrase 2 Guinier plot ln 4.06×103 Rg: 1.9 nm 0 (1.9 nm)-2 s2
(sRg)2I(s)/I(0)
Carbonic anhydrase 2 Kratky plot 1.104 0 3 sRg

Data validation


Fits and models


log I(s)
 s, nm-1
Carbonic anhydrase 2 PDB (PROTEIN DATA BANK) model

Synchrotron SAXS data from solutions of carbonic anhydrase in Tbs, pH 7.5 were collected on the EMBL-P12 beam line at the PETRA III storage ring (Hamburg, Germany), using a Pilatus 2M detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.062 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 17.00 mg/ml was measured at 20°C. 35 successive 0.100 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the data scaled to protein concentration.

Tags: benchmark
Carbonic anhydrase 2
Mol. type   Protein
Organism   Bos taurus
Olig. state   Monomer
Mon. MW   29.0 kDa
 
UniProt   P00921
Sequence   FASTA
 
PDB code   1V9E