SASDCG8 – Bovine carbonic anhydrase (WAXS)

Carbonic anhydrase 2

MW^experimental	26	kDa
MW^expected	29	kDa

log I(s) 4.05×10³ 4.05×10² 4.05×10¹ 4.05×10⁰

Carbonic anhydrase 2 small angle scattering data

s, nm^-1

Log plot Log-Log plot

ln I(s)

ln 4.06×10³ R_g: 1.9 nm 0 (1.9 nm)^-2 s²

(sR_g)²I(s)/I(0)

1.104 0 √3 sR_g

Data validation

Fits and models

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

Carbonic anhydrase 2 PDB (PROTEIN DATA BANK) model

Synchrotron SAXS data from solutions of carbonic anhydrase in Tbs, pH 7.5 were collected on the EMBL-P12 beam line at the PETRA III storage ring (Hamburg, Germany), using a Pilatus 2M detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.062 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 17.00 mg/ml was measured at 20°C. 35 successive 0.100 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the data scaled to protein concentration.

Tags: benchmark

Carbonic anhydrase 2
Mol. type		Protein
Organism		Bos taurus
Olig. state		Monomer
Mon. MW		29.0 kDa

UniProt		P00921
Sequence		FASTA

PDB ID		1V9E