WAXS benchmark on standard proteins

Maxim Petoukhov.

SASDCH8 – Horse heart cytochrome C (WAXS)

Cytochrome c
MWexperimental 7 kDa
MWexpected 12 kDa
log I(s) 1.16×104 1.16×103 1.16×102 1.16×101
Cytochrome c small angle scattering data  s, nm-1
ln I(s)
Cytochrome c Guinier plot ln 1.17×104 Rg: 1.2 nm 0 (1.2 nm)-2 s2
Cytochrome c Kratky plot 1.104 0 3 sRg

Data validation

Fits and models

log I(s)
 s, nm-1
Cytochrome c PDB (PROTEIN DATA BANK) model

Synchrotron SAXS data from solutions of horse heart cytochrome C in tbs, pH 7.5 were collected on the EMBL-P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.062 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 2.60 mg/ml was measured at 20°C. 34 successive 0.100 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the scaled to protein concentration.

Tags: benchmark
Cytochrome c
Mol. type   Protein
Organism   Equus caballus
Olig. state   Monomer
Mon. MW   11.7 kDa
UniProt   P00004
Sequence   FASTA