WAXS benchmark on standard proteins

Maxim Petoukhov.

SASDCJ8 – Glucose isomerase (WAXS)

Xylose isomerase
MWexperimental 151 kDa
MWexpected 172 kDa
log I(s) 7.95×103 7.95×102 7.95×101 7.95×100
Xylose isomerase small angle scattering data  s, nm-1
ln I(s)
Xylose isomerase Guinier plot ln 7.95×103 Rg: 3.2 nm 0 (3.2 nm)-2 s2
Xylose isomerase Kratky plot 1.104 0 3 sRg

Data validation

Fits and models

log I(s)
 s, nm-1
Xylose isomerase PDB (PROTEIN DATA BANK) model

Synchrotron SAXS data from solutions of glucose isomerase (a.k.a. xylose isomerase) in 100 mM Tris, 100 mM NaCl, 1 mM MgCl2, pH 8 were collected on the EMBL-P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 2M detector at a wavelength of λ = 0.062 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Solute concentrations ranging between 3.6 and 14.5 mg/ml were measured at 10°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Sample detector distance = UNKNOWN. Number of frames = UNKNOWN

Tags: benchmark
Xylose isomerase (GI)
Mol. type   Protein
Organism   Streptomyces rubiginosus
Olig. state   Tetramer
Mon. MW   43.1 kDa
UniProt   P24300
Sequence   FASTA