High-resolution structure of the alcohol dehydrogenase domain of the bifunctional bacterial enzyme AdhE.

Azmi L, Bragginton EC, Cadby IT, Byron O, Roe AJ, Lovering AL, Gabrielsen M, Acta Crystallogr F Struct Biol Commun 76(Pt 9):414-421 (2020) Europe PMC

SASDCK3 – Aldehyde dehydrogenase

Aldehyde-alcohol dehydrogenase
MWexperimental 47 kDa
MWexpected 48 kDa
VPorod 74 nm3
log I(s) 1.80×10-1 1.80×10-2 1.80×10-3 1.80×10-4
Aldehyde-alcohol dehydrogenase small angle scattering data  s, nm-1
ln I(s)
Aldehyde-alcohol dehydrogenase Guinier plot ln 1.80×10-1 Rg: 2.7 nm 0 (2.7 nm)-2 s2
Aldehyde-alcohol dehydrogenase Kratky plot 1.104 0 3 sRg
Aldehyde-alcohol dehydrogenase pair distance distribution function Rg: 2.7 nm 0 Dmax: 11.1 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Aldehyde-alcohol dehydrogenase DAMMIF model

log I(s)
 s, nm-1
Aldehyde-alcohol dehydrogenase DAMFILT model

Synchrotron SAXS data from solutions of aldehyde dehydrogenase in 30 mM HEPES, 150 mM NaCl, 5% (v/v) glycerol, pH 7.5 were collected on the B21 camera at the Diamond Light Source (Oxfordshire, UK) using a Pilatus 2M detector at a sample-detector distance of 4.0 m and at a wavelength of λ = 0.1 nm ((Is) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 2.00 mg/ml was measured at 4°C. 18 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged and the scattering of the solvent-blank was subtracted to obtain the SAXS profile displayed in this entry.

Aldehyde dehydrogenase (AldDH) is the N-terminal domain of the bifunctional alcohol/aldehyde dehydrogenase (AdhE; SASBDB entry SASDC72) spanning amino acids 1-445 of the full-length protein. SAXS data were measured from a protein construct that included a C-terminal 6 x histidine affinity chromatography tag. The buffer subtraction was performed using Scatter. Subsequent analysis was performed using PRIMUS (ATSAS 2.7.1).

Aldehyde-alcohol dehydrogenase (AdhE)
Mol. type   Protein
Organism   Escherichia coli O157:H7
Olig. state   Monomer
Mon. MW   48.0 kDa
UniProt   P0A9Q8 (1-445)
Sequence   FASTA