Lys63-linked ubiquitin chain adopts multiple conformational states for specific target recognition.

Liu Z, Gong Z, Jiang WX, Yang J, Zhu WK, Guo DC, Zhang WP, Liu ML, Tang C, Elife 4 (2015) Europe PMC

SASDCL6 – Lys63-linked dimer ubiquitin

MWexperimental 14 kDa
MWexpected 17 kDa
VPorod 24 nm3
log I(s) 6.90×101 6.90×100 6.90×10-1 6.90×10-2
Polyubiquitin-C small angle scattering data  s, nm-1
ln I(s)
Polyubiquitin-C Guinier plot ln 6.91×101 Rg: 2.1 nm 0 (2.1 nm)-2 s2
Polyubiquitin-C Kratky plot 1.104 0 3 sRg
Polyubiquitin-C pair distance distribution function Rg: 2.1 nm 0 Dmax: 6.5 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of 0.3mM K63-diUb in 100mM NaCl, 10mM sodium acetate, pH 6 were collected on the BL19U2 camera on the storage ring Shanghai Synchrotron Radiation Facility (SSRF) (Shanghai, China). Using a Pilatus 1M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.1033 nm one solute concentration of 5.1 mg/ml was measured at 25°C. 20 successive 1 second frames were collected, the data were normalized to the intensity of the transmitted beam and radially averaged, the scattering of the solvent-blank was subtracted. The angular range between 0.271 and 2.282 1/nm was used for PDDF conversion.

Polyubiquitin-C (ubiquitin)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   8.6 kDa
UniProt   P0CG48 (305-379)
Sequence   FASTA