A high flux setup for millisecond-scale small-angle X-ray scattering studies on macromolecular solutions

Clement Blanchet.

SASDCS8 – Cytochrome C

Cytochrome C
MWexperimental 8 kDa
MWexpected 12 kDa
log I(s) 1.19×106 1.19×105 1.19×104 1.19×103
Cytochrome C small angle scattering data  s, nm-1
ln I(s)
Cytochrome C Guinier plot ln 1.20×106 Rg: 1.3 nm 0 (1.3 nm)-2 s2
Cytochrome C Kratky plot 1.104 0 3 sRg

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of cytochrome C in 50 mM Tris, pH 7.4 were collected at the EMBL-P12 beam line at the PETRA III storage ring (Hamburg, Germany) using an Eiger 4M detector at a sample-detector distance of 1.6 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 2.00 mg/ml was measured at 20°C. A single 1.3 millisecond frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the data were scaled to the protein concentration.

Tags: benchmark
Cytochrome C (CytC)
Mol. type   Protein
Organism   Bos taurus
Olig. state   Monomer
Mon. MW   11.7 kDa
UniProt   P62894
Sequence   FASTA