| MWI(0) | 90 | kDa |
| MWexpected | 62 | kDa |
| VPorod | 145 | nm3 |
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log I(s)
2.36×103
2.36×102
2.36×101
2.36×100
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s, nm-1
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Prp19/Pso4 Is an Autoinhibited Ubiquitin Ligase Activated by Stepwise Assembly of Three Splicing Factors.
de Moura TR, Mozaffari-Jovin S, Szabó CZK, Schmitzová J, Dybkov O, Cretu C, Kachala M, Svergun D, Urlaub H, Lührmann R, Pena V, Mol Cell 69(6):979-992.e6 (2018) Europe PMCSASDCT8 – Uncharacterized protein CTHT_0072540 (Core) from Chaetomium thermophilum
hypothetical protein CTHT_0072540|
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Fits and models
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Synchrotron SAXS data from solutions of uncharacterized protein CTHT_0072540 (Core) from Chaetomium thermophilum in 20 mM HEPES, 100 mM NaCl, 2 mM β-mercaptoethanol, pH 7.5, were collected on the P12 beam line at the PETRAIII the storage ring (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Solute concentrations ranging between 1.3 and 5 mg/ml were measured at 25°C. 20 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration.
Storage temperature = UNKNOWN |
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