Prp19/Pso4 Is an Autoinhibited Ubiquitin Ligase Activated by Stepwise Assembly of Three Splicing Factors.

de Moura TR, Mozaffari-Jovin S, Szabó CZK, Schmitzová J, Dybkov O, Cretu C, Kachala M, Svergun D, Urlaub H, Lührmann R, Pena V, Mol Cell 69(6):979-992.e6 (2018) PubMed

SASDCV8 – Uncharacterized protein CTHT_0072540 (Prp19 full-length) from Chaetomium thermophilum

Full-length hypothetical protein CTHT_0072540
MWI(0) 220 kDa
MWexpected 207 kDa
VPorod 280 nm3
log I(s) 8.40×10-4 8.40×10-5 8.40×10-6 8.40×10-7
Full-length hypothetical protein CTHT_0072540 small angle scattering data  s, nm-1
ln I(s)
Full-length hypothetical protein CTHT_0072540 Guinier plot ln 8.40×10-4 Rg: 6.2 nm 0 (6.2 nm)-2 s2
(sRg)2I(s)/I(0)
Full-length hypothetical protein CTHT_0072540 Kratky plot 1.104 0 3 sRg
p(r)
Full-length hypothetical protein CTHT_0072540 pair distance distribution function Rg: 6.7 nm 0 Dmax: 23 nm

Experimental data validation


Fits and models


log I(s)
 s, nm-1
Full-length hypothetical protein CTHT_0072540 SASREF model
log I(s)
 s, nm-1
Full-length hypothetical protein CTHT_0072540 DAMMIF model
Synchrotron SAXS data from solutions of Uncharacterized protein CTHT_0072540 (Prp19 full-length) from Chaetomium thermophilum in 20 mM HEPES, 100 mM NaCl, 2 mM β-mercaptoethanol, pH 7.5, were collected on the I911-4 beam line at the MAX IV storage ring (Lund, Sweden) using a Pilatus 1M detector at the wavelength λ = 0.091 nm and a sample-detector distance of 2.2 m (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Solute concentrations ranging between 0.6 and 4.4 mg/ml were measured at 25°C with an exposure time of 120 seconds. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Full-length hypothetical protein CTHT_0072540
Mol. type   Protein
Organism   Chaetomium thermophilum
Olig. state   Tetramer
Mon. MW   51.8 kDa
 
UniProt   G0SFY0 (1-480)
Sequence   FASTA