Rev7 dimerization is important for assembly and function of the Rev1/PolĪ¶ translesion synthesis complex.

Rizzo AA, Vassel FM, Chatterjee N, D'Souza S, Li Y, Hao B, Hemann MT, Walker GC, Korzhnev DM, Proc Natl Acad Sci U S A 115(35):E8191-E8200 (2018) Europe PMC

SASDCW8 – Human Rev7 monomer (R124A mutant) in complex with Rev3 peptide @ 4mg/mL

Mitotic spindle assembly checkpoint protein MAD2B
DNA polymerase zeta catalytic subunit
MWI(0) 27 kDa
MWexpected 28 kDa
VPorod 46 nm3
log I(s) 6.84×10-2 6.84×10-3 6.84×10-4 6.84×10-5
Mitotic spindle assembly checkpoint protein MAD2B DNA polymerase zeta catalytic subunit small angle scattering data  s, nm-1
ln I(s)
Mitotic spindle assembly checkpoint protein MAD2B DNA polymerase zeta catalytic subunit Guinier plot ln 6.84×10-2 Rg: 2.1 nm 0 (2.1 nm)-2 s2
(sRg)2I(s)/I(0)
Mitotic spindle assembly checkpoint protein MAD2B DNA polymerase zeta catalytic subunit Kratky plot 1.104 0 3 sRg
p(r)
Mitotic spindle assembly checkpoint protein MAD2B DNA polymerase zeta catalytic subunit pair distance distribution function Rg: 2.0 nm 0 Dmax: 6.5 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Mitotic spindle assembly checkpoint protein MAD2B DNA polymerase zeta catalytic subunit PDB model

Synchrotron SAXS data from solutions of Human Rev7 monomer (R124A mutant) in complex with Rev3 peptide @ 4mg/mL in 20 mM Tris, 150 mM NaCl, 1 mM EDTA, 10 mM DTT, 5% glycerol, pH 8.4 were collected on the G1 beam line at the Cornell High Energy Synchrotron Source (CHESS) storage ring (Ithaca, NY, USA) using a Finger Lakes CCD detector at a wavelength of λ = 0.1267 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 4.00 mg/ml was measured at 4°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Mitotic spindle assembly checkpoint protein MAD2B (hRev7-R124A)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   24.3 kDa
 
UniProt   Q9UI95 (1-211)
Sequence   FASTA
 
DNA polymerase zeta catalytic subunit (Rev3-RBM2)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   3.3 kDa
 
UniProt   O60673 (1988-2014)
Sequence   FASTA
 
PDB code   6BC8