Structural changes of tailless bacteriophage ΦX174 during penetration of bacterial cell walls.

Sun Y, Roznowski AP, Tokuda JM, Klose T, Mauney A Pollack L, Fane BA, Rossmann MG, Proc Natl Acad Sci U S A 114(52):13708-13713 (2017) Europe PMC

SASDCW9 – Bacteriophage phi-X174 - ~245s after mixing with LPS

Bacteriophage phi-X174
MWexpected 0 kDa
log I(s) 3.56×101 3.56×100 3.56×10-1 3.56×10-2
Bacteriophage phi-X174 small angle scattering data  s, nm-1
ln I(s)
Bacteriophage phi-X174 Guinier plot ln 3.56×101 Rg: 12.9 nm 0 (12.9 nm)-2 s2
(sRg)2I(s)/I(0)
Bacteriophage phi-X174 Kratky plot 1.104 0 √3 sRg

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Bacteriophage phi-X174 ~245 s after mixing with Lipopolysaccharide (LPS) at 0.15 mg/mL in 0.06 M NH4Cl2, 0.09 M NaCl, 0.1 M KCl, 1 mM MgS04, 1 mM CaCl2, 0.1 M Tris-HCl, pH 7.4 were collected on the BioSAXS G1 beam line at the Cornell High Energy Synchrotron Source (CHESS; Ithaca, NY, USA) using a Pilatus Pilatus 200K detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.1109 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 0.10 mg/ml phage was measured at 33°C. 10 successive 10 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Note: Due to the size of the capsids, the Rg and I(0) obtained from Guinier analysis should be interpreted as approximated estimates only.

Bacteriophage phi-X174
Mol. type   Protein
Olig. state   Monomer
Mon. MW   0.2 kDa
Sequence   FASTA