Cryptic Disorder Out of Disorder: Encounter between Conditionally Disordered CP12 and Glyceraldehyde-3-Phosphate Dehydrogenase.

Launay H, Barré P, Puppo C, Zhang Y, Maneville S, Gontero B, Receveur-Bréchot V, J Mol Biol 430(8):1218-1234 (2018) Europe PMC

SASDCY9 – Oxidised chloroplastic calvin cycle protein CP12 from C. reinhardtii

Calvin cycle protein CP12, chloroplastic
MWI(0) 13 kDa
MWexpected 11 kDa
VPorod 22 nm3
log I(s) 3.90×10-3 3.90×10-4 3.90×10-5 3.90×10-6
Calvin cycle protein CP12, chloroplastic small angle scattering data  s, nm-1
ln I(s)
Calvin cycle protein CP12, chloroplastic Guinier plot ln 3.90×10-3 Rg: 2.3 nm 0 (2.3 nm)-2 s2
(sRg)2I(s)/I(0)
Calvin cycle protein CP12, chloroplastic Kratky plot 1.104 0 3 sRg
p(r)
Calvin cycle protein CP12, chloroplastic pair distance distribution function Rg: 2.5 nm 0 Dmax: 10 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Oxidised chloroplastic calvin cycle protein CP12 from C. reinhardtii Rg histogram Rg, nm

log I(s)
 s, nm-1
Calvin cycle protein CP12, chloroplastic GASBOR model

log I(s)
 s, nm-1
Calvin cycle protein CP12, chloroplastic DAMFILT model

Synchrotron SAXS data from solutions of oxidised chloroplastic calvin cycle protein CP12i in 50 mM phosphate buffer, 50 mM NaCl, 20 mM oxidized DTT, pH 6.5, were collected on the SWING beam line at the SOLEIL storage ring (Saint-Aubin, France) using a CCD AVIEX detector at a sample-detector distance of 1.8 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 0.54 mg/ml was measured at 20°C. 250 successive 1.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the data were scaled for protein concentration.

Tags: idp
Calvin cycle protein CP12, chloroplastic (CP12)
Mol. type   Protein
Organism   Chlamydomonas reinhardtii
Olig. state   Monomer
Mon. MW   10.9 kDa
 
UniProt   A6Q0K5 (28-107)
Sequence   FASTA