Cryptic Disorder Out of Disorder: Encounter between Conditionally Disordered CP12 and Glyceraldehyde-3-Phosphate Dehydrogenase.

Launay H, Barré P, Puppo C, Zhang Y, Maneville S, Gontero B, Receveur-Bréchot V, J Mol Biol 430(8):1218-1234 (2018) PubMed

SASDD22 – Glyceraldehyde-3-phosphate dehydrogenase in complex with oxidised chloroplastic calvin cycle protein CP12 (C. reinhardtii)

Calvin cycle protein CP12, chloroplastic
Glyceraldehyde-3-phosphate dehydrogenase
MWexperimental 166 kDa
MWexpected 158 kDa
VPorod 251 nm3
log I(s) 3.85×10-4 3.85×10-5 3.85×10-6 3.85×10-7
Calvin cycle protein CP12, chloroplastic Glyceraldehyde-3-phosphate dehydrogenase small angle scattering data  s, nm-1
ln I(s)
Calvin cycle protein CP12, chloroplastic Glyceraldehyde-3-phosphate dehydrogenase Guinier plot ln 3.85×10-4 Rg: 3.8 nm 0 (3.8 nm)-2 s2
(sRg)2I(s)/I(0)
Calvin cycle protein CP12, chloroplastic Glyceraldehyde-3-phosphate dehydrogenase Kratky plot 1.104 0 3 sRg
p(r)
Calvin cycle protein CP12, chloroplastic Glyceraldehyde-3-phosphate dehydrogenase pair distance distribution function Rg: 4.0 nm 0 Dmax: 19 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Glyceraldehyde-3-phosphate dehydrogenase in complex with oxidised chloroplastic calvin cycle protein CP12 (C. reinhardtii) Rg histogram Rg, nm

log I(s)
 s, nm-1
Glyceraldehyde-3-phosphate dehydrogenase in complex with oxidised chloroplastic calvin cycle protein CP12 (C. reinhardtii) Rg histogram Rg, nm

Synchrotron SAXS data from solutions of Glyceraldehyde-3-phosphate dehydrogenase in complex with oxidised chloroplastic calvin cycle protein CP12 in 30 mM Tris, 4 mM EDTA, 100 µM NAD, 5 mM free cysteine, pH 7.9 were collected on the SWING beam line at the SOLEIL storage ring (Saint-Aubin, France) using a CCD AVIEX detector at a sample-detector distance of 1.8 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 16.00 mg/ml was measured at 20°C. 250 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration.

Calvin cycle protein CP12, chloroplastic (CP12)
Mol. type   Protein
Organism   Chlamydomonas reinhardtii
Olig. state   Monomer
Mon. MW   10.9 kDa
 
UniProt   A6Q0K5 (28-107)
Sequence   FASTA
 
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
Mol. type   Protein
Organism   Chlamydomonas reinhardtii
Olig. state   Tetramer
Mon. MW   36.9 kDa
 
UniProt   A8HP84
Sequence   FASTA