Insights into catalysis and function of phosphoribosyl-linked serine ubiquitination.

Kalayil S, Bhogaraju S, Bonn F, Shin D, Liu Y, Gan N, Basquin J, Grumati P, Luo ZQ, Dikic I, Nature 557(7707):734-738 (2018) PubMed

SASDD65 – Ubiquitinating/deubiquitinating enzyme SdeA 207 914

Ubiquitinating/deubiquitinating enzyme SdeA
MWI(0) 80 kDa
MWexpected 72 kDa
VPorod 128 nm3
log I(s) 5.70×10-2 5.70×10-3 5.70×10-4 5.70×10-5
Ubiquitinating/deubiquitinating enzyme SdeA small angle scattering data  s, nm-1
ln I(s)
Ubiquitinating/deubiquitinating enzyme SdeA Guinier plot ln 5.70×10-2 Rg: 3.5 nm 0 (3.5 nm)-2 s2
(sRg)2I(s)/I(0)
Ubiquitinating/deubiquitinating enzyme SdeA Kratky plot 1.104 0 3 sRg
p(r)
Ubiquitinating/deubiquitinating enzyme SdeA pair distance distribution function Rg: 3.5 nm 0 Dmax: 11.2 nm

Experimental data validation


Fits and models


log I(s)
 s, nm-1
Ubiquitinating/deubiquitinating enzyme SdeA OTHER model
log I(s)
 s, nm-1
Ubiquitinating/deubiquitinating enzyme SdeA DAMMIN model
Synchrotron SAXS data from solutions of Ubiquitinating/deubiquitinating enzyme, SdeA, in 10 mM HEPES 150 mM NaCl 1 mM TCEP, pH 7.5, were collected on the EMBL-P12 bioSAXS beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.64 mg/ml was measured at 10°C. 20 successive 0.045 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Ubiquitinating/deubiquitinating enzyme SdeA (SdeA)
Mol. type   Protein
Organism   Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513)
Olig. state   Monomer
Mon. MW   71.8 kDa
 
UniProt   Q5ZTK4 (277-907)
Sequence   FASTA