'TG' interacting factor

Ewelina Guca.

SASDD75 – 'TG'-interacting factor homeodomain in solution (Homeobox protein TGIF1)

Homeobox protein TGIF1
MWexperimental 26 kDa
MWexpected 23 kDa
VPorod 42 nm3
log I(s) 1.14×104 1.14×103 1.14×102 1.14×101
Homeobox protein TGIF1 small angle scattering data  s, nm-1
ln I(s)
Homeobox protein TGIF1 Guinier plot ln 1.14×104 Rg: 2.7 nm 0 (2.7 nm)-2 s2
(sRg)2I(s)/I(0)
Homeobox protein TGIF1 Kratky plot 1.104 0 √3 sRg
p(r)
Homeobox protein TGIF1 pair distance distribution function Rg: 2.8 nm 0 Dmax: 9.2 nm

Data validation

Fits and models

log I(s)
 s, nm-1
'TG'-interacting factor homeodomain in solution (Homeobox protein TGIF1) Rg histogram Rg, nm
Homeobox protein TGIF1 EOM/RANCH model
Homeobox protein TGIF1 EOM/RANCH model
Homeobox protein TGIF1 EOM/RANCH model
log I(s)
 s, nm-1
Homeobox protein TGIF1 DAMMIF model
log I(s)
 s, nm-1
Homeobox protein TGIF1 MODLOOPS model
Synchrotron SAXS data from solutions of 'TG'-interacting factor homeodomain in solution (Homeobox protein TGIF1) in 20 mM Tris, 150 mM NaCl, pH 7.4 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 6.00 mg/ml was measured at 20°C. 120 successive 0.045 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Homeobox protein TGIF1 (TGIF)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   11.7 kDa
 
UniProt   Q15583 (151-248)
Sequence   FASTA
 
PDB ID   6FQQ
 
PDB ID   6FQQ
 
PDB ID   6FQQ
 
PDB ID   6FQQ