Functionally specific binding regions of microtubule-associated protein 2c exhibit distinct conformations and dynamics.

Melková K, Zapletal V, Jansen S, Nomilner E, Zachrdla M, Hritz J, Nováček J, Zweckstetter M, Jensen MR, Blackledge M, Žídek L, J Biol Chem 293(34):13297-13309 (2018) Europe PMC

SASDDB2 – Phosphorylated Microtubule associated protein MAP2c (isoform 3); 6.8 mg/ml

Microtubule-associated protein 2, isoform 3
MWexperimental 66 kDa
MWexpected 49 kDa
log I(s) 3.25×101 3.25×100 3.25×10-1 3.25×10-2
Microtubule-associated protein 2, isoform 3 small angle scattering data  s, nm-1
ln I(s)
Microtubule-associated protein 2, isoform 3 Guinier plot ln 3.25×101 Rg: 7.3 nm 0 (7.3 nm)-2 s2
Microtubule-associated protein 2, isoform 3 Kratky plot 1.104 0 3 sRg

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of phosphorylated microtubule associated protein MAP2c (isoform 3) in 50 mM MOPS, 150 mM NaCl, 0,03% NaN3, pH 6.9 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at an X-ray wavelength, λ, of 0.099 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 6.80 mg/ml was measured at 20°C. A total of 10 successive 1 s data frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the data were scaled for protein concentration.

Tags: idp
Microtubule-associated protein 2, isoform 3 (MAP2c)
Mol. type   Protein
Organism   Rattus norvegicus
Olig. state   Monomer
Mon. MW   49.3 kDa
UniProt   P15146-3
Sequence   FASTA