Structure and oligomerization of the periplasmic domain of GspL from the type II secretion system of Pseudomonas aeruginosa

Fulara A, Vandenberghe I, Read R, Devreese B, Savvides S, Scientific Reports 8(1) (2018) DOI

SASDDB8 – Periplasmic domain of inner membrane protein GspL, dimer

Type II secretion system protein L, periplasmic domain
MWI(0) 36 kDa
MWexpected 28 kDa
log I(s) 3.91×10-2 3.91×10-3 3.91×10-4 3.91×10-5
Type II secretion system protein L, periplasmic domain small angle scattering data  s, nm-1
ln I(s)
Type II secretion system protein L, periplasmic domain Guinier plot ln 3.91×10-2 Rg: 2.2 nm 0 (2.2 nm)-2 s2
(sRg)2I(s)/I(0)
Type II secretion system protein L, periplasmic domain Kratky plot 1.104 0 3 sRg
p(r)
Type II secretion system protein L, periplasmic domain pair distance distribution function Rg: 2.2 nm 0 Dmax: 7.5 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Type II secretion system protein L, periplasmic domain EOM/RANCH model
Type II secretion system protein L, periplasmic domain EOM/RANCH model
Type II secretion system protein L, periplasmic domain EOM/RANCH model
Type II secretion system protein L, periplasmic domain EOM/RANCH model
Type II secretion system protein L, periplasmic domain EOM/RANCH model
Type II secretion system protein L, periplasmic domain EOM/RANCH model

Synchrotron SAXS data from solutions of Periplasmic domain of inner membrane protein GspL, dimer in 50 mM TRIS, 100 mM NaCl, pH 7.5 were collected on the SWING camera at the SOLEIL storage ring (Saint-Aubin, France) using a CCD AVIEX PCCD170170 detector at a wavelength of λ = 0.1033 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 2 and 13.7 mg/ml were measured at 15°C. 19 successive frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Type II secretion system protein L, periplasmic domain (GspLperi)
Mol. type   Protein
Organism   Pseudomonas aeruginosa
Olig. state   Dimer
Mon. MW   14.0 kDa
 
UniProt   P25060 (261-382)
Sequence   FASTA