OCP-FRP protein complex topologies suggest a mechanism for controlling high light tolerance in cyanobacteria.

Sluchanko NN, Slonimskiy YB, Shirshin EA, Moldenhauer M, Friedrich T, Maksimov EG, Nat Commun 9(1):3869 (2018) PubMed

SASDDG9 – The 2:1 complex of Synechocystis disulphide-trapped Fluorescence recovery protein dimer (CC mutant) and orange carotenoid-binding protein-ΔNTE (orange form)

Fluorescence recovery protein
Orange carotenoid-binding protein
MWexperimental 64 kDa
MWexpected 62 kDa
VPorod 102 nm3
log I(s) 3.21×10-2 3.21×10-3 3.21×10-4 3.21×10-5
Fluorescence recovery protein Orange carotenoid-binding protein small angle scattering data  s, nm-1
ln I(s)
Fluorescence recovery protein Orange carotenoid-binding protein Guinier plot ln 3.22×10-2 Rg: 3.0 nm 0 (3.0 nm)-2 s2
(sRg)2I(s)/I(0)
Fluorescence recovery protein Orange carotenoid-binding protein Kratky plot 1.104 0 3 sRg
p(r)
Fluorescence recovery protein Orange carotenoid-binding protein pair distance distribution function Rg: 3.2 nm 0 Dmax: 13 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Fluorescence recovery protein Orange carotenoid-binding protein GASBOR model

log I(s)
 s, nm-1
Fluorescence recovery protein Orange carotenoid-binding protein GASBOR model

log I(s)
 s, nm-1
Fluorescence recovery protein Orange carotenoid-binding protein CORAL model

Synchrotron SAXS data from solutions of the 2:1 complex of the disulfide trapped FRP dimer (CC) and OCP-deltaNTE mutant of the Synechocystis OCP (orange form) in 20 mM Tris-HCl, 150 mM NaCl, 0.1 mM EDTA, 3 % v/v glycerol, pH 7.6 were collected at the EMBL-P12 bioSAXS beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 2M detector at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 2.41 mg/ml was measured at 10°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the data scaled for protein concentration.

Fluorescence recovery protein
Mol. type   Protein
Organism   Synechocystis sp. (strain PCC 6803 / Kazusa)
Olig. state   Dimer
Mon. MW   14.1 kDa
 
UniProt   P74103
Sequence   FASTA
 
Orange carotenoid-binding protein (OCP)
Mol. type   Protein
Organism   Synechocystis sp. (strain PCC 6803 / Kazusa)
Olig. state   Monomer
Mon. MW   34.3 kDa
 
UniProt   P74102
Sequence   FASTA