The X-ray structure of human calbindin-D28K: an improved model

Noble J, Almalki R, Roe S, Wagner A, Duman R, Atack J, Acta Crystallographica Section D Structural Biology 74(10):1008-1014 (2018) DOI

SASDDM6 – Calbindin-D28K

MWexperimental 25 kDa
MWexpected 30 kDa
VPorod 46 nm3
log I(s) 8.56×10-2 8.56×10-3 8.56×10-4 8.56×10-5
Calbindin small angle scattering data  s, nm-1
ln I(s)
Calbindin Guinier plot ln 8.56×10-2 Rg: 2.1 nm 0 (2.1 nm)-2 s2
Calbindin Kratky plot 1.104 0 3 sRg
Calbindin pair distance distribution function Rg: 2.1 nm 0 Dmax: 7.0 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Calbindin DAMMIF model

Synchrotron SAXS data from solutions of calbindin-D28K in 20 mM Tris, 150mM NaCl, pH 7.8, were collected on the B21 beam line at the Diamond Light Source (Oxfordshire, UK) using a Pilatus 2M detector at a sample-detector distance of 4 m and at a wavelength of λ = 0.1 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 12.20 mg/ml was measured at 25°C. 14 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Calbindin (CALB1)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   30.3 kDa
UniProt   P05937
Sequence   FASTA