Stability of local secondary structure determines selectivity of viral RNA chaperones.

Bravo JPK, Borodavka A, Barth A, Calabrese AN, Mojzes P, Cockburn JJB, Lamb DC, Tuma R, Nucleic Acids Res (2018) Europe PMC

SASDDT5 – Ribonucleoprotein complex of nonstructural protein sigma NS bound to 20mer RNA (NS-RNP20)

Nonstructural protein sigma NS
20mer RNA (unstructured)
MWexperimental 323 kDa
MWexpected 337 kDa
VPorod 964 nm3
log I(s) 2.99×10-1 2.99×10-2 2.99×10-3 2.99×10-4
Nonstructural protein sigma NS 20mer RNA (unstructured) small angle scattering data  s, nm-1
ln I(s)
Nonstructural protein sigma NS 20mer RNA (unstructured) Guinier plot ln 2.99×10-1 Rg: 7.8 nm 0 (7.8 nm)-2 s2
(sRg)2I(s)/I(0)
Nonstructural protein sigma NS 20mer RNA (unstructured) Kratky plot 1.104 0 √3 sRg
p(r)
Nonstructural protein sigma NS 20mer RNA (unstructured) pair distance distribution function Rg: 8.7 nm 0 Dmax: 38 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of ribonucleoprotein complex of nonstructural protein sigma NS bound to 20mer RNA (NS-RNP20) in 25 mM HEPES, 150 mM NaCl, pH 7.5 were collected on the B21 beam line at the Diamond Light Source storage ring (Oxfordshire, UK) using a Pilatus 2M detector at a sample-detector distance of 3.9 m and at a wavelength of λ = 0.1 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

SigmaNS ribonucleoprotein complex with 20mer RNA. Sample concentration, UNKNOWN; Sample temperature, UNKNOWN; Exposure time; UNKNOWN.

Nonstructural protein sigma NS
Mol. type   Protein
Organism   Avian orthoreovirus
Olig. state   Octamer
Mon. MW   40.6 kDa
 
UniProt   Q9DH28
Sequence   FASTA
 
20mer RNA (unstructured) (20mer)
Mol. type   RNA
Olig. state   Dimer
Mon. MW   6.4 kDa
Sequence   FASTA