Stability of local secondary structure determines selectivity of viral RNA chaperones.

Bravo JPK, Borodavka A, Barth A, Calabrese AN, Mojzes P, Cockburn JJB, Lamb DC, Tuma R, Nucleic Acids Res (2018) Europe PMC

SASDDU5 – Nonstructural protein sigma NS - apoprotein

Nonstructural protein sigma NS
MWI(0) 240 kDa
MWexpected 244 kDa
VPorod 670 nm3
log I(s) 3.19×10-1 3.19×10-2 3.19×10-3 3.19×10-4
Nonstructural protein sigma NS small angle scattering data  s, nm-1
ln I(s)
Nonstructural protein sigma NS Guinier plot ln 3.19×10-1 Rg: 5.5 nm 0 (5.5 nm)-2 s2
(sRg)2I(s)/I(0)
Nonstructural protein sigma NS Kratky plot 1.104 0 √3 sRg
p(r)
Nonstructural protein sigma NS pair distance distribution function Rg: 5.7 nm 0 Dmax: 23.1 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of nonstructural protein sigma NS (apoprotein) in 25 mM HEPES, 150 mM NaCl, pH 7.5 were collected on the B21 beam line at the Diamond Light Source storage ring (Oxfordshire, UK) using a Pilatus 2M detector at a sample-detector distance of 3.9 m and at a wavelength of λ = 0.1 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Sample concentration, UNKNOWN; Sample temperature, UNKNOWN; Exposure time; UNKNOWN.

Nonstructural protein sigma NS
Mol. type   Protein
Organism   Avian orthoreovirus
Olig. state   Hexamer
Mon. MW   40.6 kDa
 
UniProt   Q9DH28
Sequence   FASTA