Synchrotron SAXS data from solutions of 4-hydroxy-tetrahydrodipicolinate synthase (DHDPS-apo) from C. botulinum in 20mM Tris, 150mM NaCl, pH 8, were collected on the SAXS/WAXS beam line at the Australian Synchrotron (Melbourne, Australia) using a Pilatus 1M detector at a sample-detector distance of 0.9 m and at a wavelength of λ = 0.10332 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). The protein sample analyzed was subjected to in-line size exclusion chromatography on a Superdex 200 5/150 GL gel-filtration column (GE Healthcare) with a bed volume of 3 ml equilibrated with buffer at a flow rate of 0.2 ml.min-1. 50 µL Cbot-DHDPS at 300 µM was injected and the fractionated sample flowed through a 1.5 mm quartz capillary where it was exposed to the X-ray beam at 25°C. Seven successive 5 second frames were collected through the SEC-elution profile. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of an appropriate SEC solvent-blank was subtracted.
s, nm-1
