Synchrotron SAXS
data from solutions of
p-hydroxyphenylacetate 3-hydroxylase, reductase component (mutant): 4 mg/ml of E248A C1 in 1 mM of p-hydroxyphenylacetic acid (HPA)
in
50 mM MOPS, 0.5 mM EDTA, 1 mM DTT, 50 mM NaCl, 1 mM HPA, 10 % glycerol, pH 7
were collected
on the
BL1.3W beam line
at the Synchrotron Light Research Institute (SLRI) storage ring
(Nakhon Ratchasima, Thailand)
using a Rayonix SX165 detector
at a sample-detector distance of 1.3 m and
at a wavelength of λ = 0.13776 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 4.00 mg/ml was measured
at 16°C.
One
600 second frame was collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
s, nm-1
