data from solutions of
p-hydroxyphenylacetate 3-hydroxylase, reductase component (mutant): 2 mg/ml of H170A C1 in the absence of p-hydroxyphenylacetic acid (HPA)
50 mM MOPS, 0.5 mM EDTA, 1 mM DTT, and 5% glycerol, pH 7
were collected on the
at the Synchrotron Light Research Institute (SLRI) storage ring
(Nakhon Ratchasima, Thailand)
using a CCD Rayonix SX165 detector
at a sample-detector distance of 1.3 m and
at a wavelength of λ = 0.13776 nm
, the range of momentum transfer 0.300 < s < 2.781 nm-1 was covered
(l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 2.00 mg/ml was measured
600 second frame was collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.