Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements.

Fuertes G, Banterle N, Ruff KM, Chowdhury A, Mercadante D, Koehler C, Kachala M, Estrada Girona G, Milles S, Mishra A, Onck PR, Gräter F, Esteban-Martín S, Pappu RV, Svergun DI, Lemke EA, Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017) Europe PMC

SASDE93 – Unlabeled thioredoxin (TRX) with denaturant

Thioredoxin 1
MWexperimental 12 kDa
MWexpected 12 kDa
VPorod 34 nm3
log I(s) 5.60×102 5.60×101 5.60×100 5.60×10-1
Thioredoxin 1 small angle scattering data  s, nm-1
ln I(s)
Thioredoxin 1 Guinier plot ln 5.61×102 Rg: 3.6 nm 0 (3.6 nm)-2 s2
(sRg)2I(s)/I(0)
Thioredoxin 1 Kratky plot 1.104 0 3 sRg
p(r)
Thioredoxin 1 pair distance distribution function Rg: 3.7 nm 0 Dmax: 13 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of Unlabeled thioredoxin (TRX) with denaturant in PBS, 10 mM DTT, 6 M urea, 0.3 M KCl, pH 7.4 were collected on the EMBL P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 10.00 mg/ml was measured at 23°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

The protein contains a penultimate non-canonical amino acid p-acetylphenylalanine (207 Da) that is represented as U (selenocysteine, 168 Da) in the amino acid sequence for the entry. Therefore, the calculated MW from sequence (MW(expected)) must be adjusted accordingly (ca. 40 Da).

Tags: idp
Thioredoxin 1 (TRX)
Mol. type   Protein
Organism   Escherichia coli
Olig. state   Monomer
Mon. MW   11.8 kDa
 
UniProt   P0AA25 (2-106)
Sequence   FASTA