Synchrotron SAXS data from solutions of polyglutamine tract-binding protein 1 (PQBP-1) in 20 mM Tris, 150 mM NaCl, 1mM DTT, pH 7 were collected on the EMBL X33 beam line at DORIS III (Hamburg, Germany) using a Pilatus 1M-W detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.15 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 7 and 13 mg/ml were measured at 20°C. Eight successive 15 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
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Polyglutamine-binding protein 1
(PQBP-1)
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Mol. type |
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Protein |
Organism |
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Homo sapiens |
Olig. state |
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Monomer |
Mon. MW |
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30.6 kDa |
|
UniProt |
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O60828
(1-265)
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Sequence |
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FASTA |
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