Migration of Type III Secretion System Transcriptional Regulators Links Gene Expression to Secretion.

Charova SN, Gazi AD, Mylonas E, Pozidis C, Sabarit B, Anagnostou D, Psatha K, Aivaliotis M, Beuzon CR, Panopoulos NJ, Kokkinidis M, MBio 9(4) (2018) PubMed

SASDEM4 – HrpG/HrpV/HrpJ gatekeeper complex from Erwinia amylovora

Type III secretion protein HrpG
Type III secretion protein HrpV
Hypersensitivity response secretion protein HrpJ
MWI(0) 75 kDa
MWexpected 75 kDa
log I(s) 2.72×10-1 2.72×10-2 2.72×10-3 2.72×10-4
Type III secretion protein HrpG Type III secretion protein HrpV Hypersensitivity response secretion protein HrpJ small angle scattering data  s, nm-1
ln I(s)
Type III secretion protein HrpG Type III secretion protein HrpV Hypersensitivity response secretion protein HrpJ Guinier plot ln 2.72×10-1 Rg: 4 nm 0 (4 nm)-2 s2
(sRg)2I(s)/I(0)
Type III secretion protein HrpG Type III secretion protein HrpV Hypersensitivity response secretion protein HrpJ Kratky plot 1.104 0 3 sRg
p(r)
Type III secretion protein HrpG Type III secretion protein HrpV Hypersensitivity response secretion protein HrpJ pair distance distribution function Rg: 4.4 nm 0 Dmax: 16 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Type III secretion protein HrpG Type III secretion protein HrpV Hypersensitivity response secretion protein HrpJ CORAL model

Synchrotron SAXS data from solutions of HrpG/HrpV/HrpJ gatekeeper complex from Erwinia amylovora in 20mM Tris-Cl, pH 8.0, 100mM NaCl, 2mM DTT, 0.5mM EDTA, pH 8 were collected on the SWING camera at the SOLEIL storage ring (Saint-Aubin, France) using a CCD AVIEX PCCD170170 detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.1033 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.50 mg/ml was measured at 15°C. 30 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Type III secretion protein HrpG
Mol. type   Protein
Organism   Erwinia amylovora (strain CFBP1430)
Olig. state   Other
Mon. MW   19.2 kDa
 
UniProt   D4HVP1
Sequence   FASTA
 
Type III secretion protein HrpV
Mol. type   Protein
Organism   Erwinia amylovora (strain CFBP1430)
Olig. state   Other
Mon. MW   13.2 kDa
 
UniProt   D4HVP4
Sequence   FASTA
 
Hypersensitivity response secretion protein HrpJ
Mol. type   Protein
Organism   Erwinia amylovora (strain CFBP1430)
Olig. state   Other
Mon. MW   42.4 kDa
 
UniProt   D4HVM8
Sequence   FASTA