Glycine decarboxylase (P-protein of glycine cleavage system)

Bart Van Laer.

SASDEM7 – Human glycine decarboxylase with glycine

Glycine decarboxylase
MWexperimental 197 kDa
MWexpected 219 kDa
VPorod 315 nm3
log I(s) 1.38×102 1.38×101 1.38×100 1.38×10-1
Glycine decarboxylase small angle scattering data  s, nm-1
ln I(s)
Glycine decarboxylase Guinier plot ln 1.38×102 Rg: 4.0 nm 0 (4.0 nm)-2 s2
(sRg)2I(s)/I(0)
Glycine decarboxylase Kratky plot 1.104 0 3 sRg

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of human glycine decarboxylase in 10 mM TRIS pH 7.5, 100 mM NaCl, 1 mM DTT , 100 mM glycine, were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 20°C. 10 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Glycine decarboxylase (GLDC)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   109.4 kDa
 
UniProt   P23378 (45-1020)
Sequence   FASTA