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Synchrotron SAXS data from solutions of Filamin A Ig-like domains 3-5 P637Q mutant in 20 mM Tris, 100 mM NaCl, 1 mM DTT, pH 8 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.1 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1 and 4 mg/ml were measured at 20°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
SAXS data describing the Filamin A Ig-like domains 3-5 P637Q mutant in solution. The experiment was done to enable comparison with the corresponding WT fragment and to validate the crystal structure of the mutant (SASDEQ7).
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s, nm-1
