KAP1 is an antiparallel dimer with a functional asymmetry.

Fonti G, Marcaida MJ, Bryan LC, Träger S, Kalantzi AS, Helleboid PJ, Demurtas D, Tully MD, Grudinin S, Trono D, Fierz B, Dal Peraro M, Life Sci Alliance 2(4) (2019) Europe PMC

SASDER7 – KRAB-associated protein 1 (KAP1); TRIM28, amino acids 23-812

Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28; amino acids 23-812
MWexperimental 290 kDa
MWexpected 175 kDa
VPorod 853 nm3
log I(s) 9.20×101 9.20×100 9.20×10-1 9.20×10-2
Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28; amino acids 23-812 small angle scattering data  s, nm-1
ln I(s)
Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28; amino acids 23-812 Guinier plot ln 9.21×101 Rg: 8.9 nm 0 (8.9 nm)-2 s2
(sRg)2I(s)/I(0)
Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28; amino acids 23-812 Kratky plot 1.104 0 3 sRg
p(r)
Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28; amino acids 23-812 pair distance distribution function Rg: 9.6 nm 0 Dmax: 38 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of KRAB-associated protein 1 (KAP1); TRIM28, amino acids 23-812 in 20 mM HEPES, 500 mM NaCl, 10 % glycerol, 2 mM TCEP, pH 7.5 were collected using size exclusion chromatography SAXS (SEC-SAXS) on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed at 20°C using the following parameters: Column type: GE Healthcare Superose 6 10/300; Flow rate: 0.5 ml/min; Injection volume: 100 µl; Total acquisition time: 60 min (3000 x 1 s frames); Injection concentration: 12 mg/mL. The data obtained through the sample elution peak were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the individual subtracted data sets were scaled and averaged to generate the scattering profile displayed in this entry.

Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28; amino acids 23-812 (KAP1, residues23-812)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   87.5 kDa
 
UniProt   Q13263 (23-812)
Sequence   FASTA