The structure of the periplasmic FlaG-FlaF complex and its essential role for archaellar swimming motility.

Tsai CL, Tripp P, Sivabalasarma S, Zhang C, Rodriguez-Franco M, Wipfler RL, Chaudhury P, Banerjee A, Beeby M, Whitaker RJ, Tainer JA, Albers SV, Nat Microbiol (2019) Europe PMC

SASDES7 – Stator protein complex FlaG/FlaF

Conserved flagellar protein F
Stator protein FlaG soluble domain
MWexperimental 57 kDa
MWexpected 62 kDa
VPorod 109 nm3
log I(s) 4.44×102 4.44×101 4.44×100 4.44×10-1
Conserved flagellar protein F Stator protein FlaG soluble domain small angle scattering data  s, nm-1
ln I(s)
Conserved flagellar protein F Stator protein FlaG soluble domain Guinier plot ln 4.44×102 Rg: 3.2 nm 0 (3.2 nm)-2 s2
(sRg)2I(s)/I(0)
Conserved flagellar protein F Stator protein FlaG soluble domain Kratky plot 1.104 0 3 sRg
p(r)
Conserved flagellar protein F Stator protein FlaG soluble domain pair distance distribution function Rg: 3.4 nm 0 Dmax: 12.5 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Conserved flagellar protein F Stator protein FlaG soluble domain MULTIFOXS model

Synchrotron SAXS data from solutions of the stator protein complex FlaG/FlaF in 25 mM citric acid/sodium citrate, 150mM NaCl, 3% glycerol, pH 3 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.127 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 2 and 6 mg/ml were measured at 10°C. 33 successive 0.300 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Conserved flagellar protein F (sFlaF)
Mol. type   Protein
Organism   Sulfolobus acidocaldarius
Olig. state   Dimer
Mon. MW   16.2 kDa
 
UniProt   Q4J9K8 (35-164)
Sequence   FASTA
 
Stator protein FlaG soluble domain (sFlaG)
Mol. type   Protein
Organism   Sulfolobus acidocaldarius
Olig. state   Dimer
Mon. MW   14.9 kDa
 
UniProt   Q4J9K7 (32-151)
Sequence   FASTA
 
PDB code   5TUG