Senescence-associated ubiquitin ligase 1 (SAUL1)

Haifa El Kilani, Al Kikhney.

SASDET3 – Armadillo repeat 7-11 from SAUL1

U-box domain-containing protein 44
MWexperimental 161 kDa
MWexpected 185 kDa
VPorod 245 nm3
log I(s) 8.87×103 8.87×102 8.87×101 8.87×100
U-box domain-containing protein 44 small angle scattering data  s, nm-1
ln I(s)
U-box domain-containing protein 44 Guinier plot ln 8.88×103 Rg: 4.5 nm 0 (4.5 nm)-2 s2
(sRg)2I(s)/I(0)
U-box domain-containing protein 44 Kratky plot 1.104 0 √3 sRg
p(r)
U-box domain-containing protein 44 pair distance distribution function Rg: 4.6 nm 0 Dmax: 17.4 nm

Data validation

Fits and models

log I(s)
 s, nm-1
U-box domain-containing protein 44 SASREF MX model
Synchrotron SAXS data from solutions of Armadillo repeat 7-11 from SAUL1 in 50 mM Tris, 250 mM NaCl, pH 9 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.123987 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.00 mg/ml was measured at 10°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

The rigid bodies used for the SASREF MX tetramer model were predicted by I-TASSER.

U-box domain-containing protein 44 (ARM)
Mol. type   Protein
Organism   Arabidopsis thaliana
Olig. state   Tetramer
Mon. MW   46.3 kDa
 
UniProt   Q9LM76 (382-801)
Sequence   FASTA