MglA functions as a three-state GTPase to control movement reversals of Myxococcus xanthus.

Galicia C, Lhospice S, Varela PF, Trapani S, Zhang W, Navaza J, Herrou J, Mignot T, Cherfils J, Nat Commun 10(1):5300 (2019) Europe PMC

SASDET9 – Gliding motility protein MglB: A GAP of Myxococcus xanthus MglA

Gliding motility protein MglB
MWI(0) 37 kDa
MWexpected 34 kDa
VPorod 56 nm3
log I(s) 4.76×10-2 4.76×10-3 4.76×10-4 4.76×10-5
Gliding motility protein MglB small angle scattering data  s, nm-1
ln I(s)
Gliding motility protein MglB Guinier plot ln 4.77×10-2 Rg: 2.8 nm 0 (2.8 nm)-2 s2
Gliding motility protein MglB Kratky plot 1.104 0 3 sRg
Gliding motility protein MglB pair distance distribution function Rg: 2.9 nm 0 Dmax: 10.3 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Gliding motility protein MglB GASBOR model

Synchrotron SAXS data from solutions of the gliding motility protein MglB in 150 mM NaCl, 1 mM DTT, 20 mM Tris-HCl, pH 8 were collected on the SWING beam line at SOLEIL (Saint-Aubin, France) using a CCD AVIEX PCCD170170 detector at a sample-detector distance of 1.8 m and at a wavelength of λ = 0.103 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 15.00 mg/ml was measured at 15°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

X-ray exposure time = UNKNOWN.

Gliding motility protein MglB (MglB)
Mol. type   Protein
Organism   Myxococcus xanthus
Olig. state   Dimer
Mon. MW   17.2 kDa
UniProt   Q1DB03 (1-159)
Sequence   FASTA