KAP1 is an antiparallel dimer with a functional asymmetry.

Fonti G, Marcaida MJ, Bryan LC, Träger S, Kalantzi AS, Helleboid PJ, Demurtas D, Tully MD, Grudinin S, Trono D, Fierz B, Dal Peraro M, Life Sci Alliance 2(4) (2019) Europe PMC

SASDEV6 – KRAB-associated protein 1 (KAP1); TRIM28; full length protein

Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28
MWexperimental 247 kDa
MWexpected 183 kDa
VPorod 710 nm3
log I(s) 7.28×101 7.28×100 7.28×10-1 7.28×10-2
Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28 small angle scattering data  s, nm-1
ln I(s)
Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28 Guinier plot ln 7.29×101 Rg: 9.0 nm 0 (9.0 nm)-2 s2
(sRg)2I(s)/I(0)
Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28 Kratky plot 1.104 0 3 sRg
p(r)
Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28 pair distance distribution function Rg: 9.5 nm 0 Dmax: 38 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28 GASBOR model

log I(s)
 s, nm-1
Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28 OTHER model

Synchrotron SAXS data from solutions of KRAB-associated protein 1 (KAP1); TRIM28; full length protein in 20 mM HEPES, 500 mM NaCl, 10 % v/v glycerol, 2 mM TCEP, pH 7.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 15.00 mg/ml was measured at 20°C for 1 second. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28 (KAP1FL)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   91.5 kDa
 
UniProt   Q13263 (1-835)
Sequence   FASTA