Identification of binding sites on human serum albumin for somapacitan - a long-acting growth hormone derivative.

Johansson E, Nielsen AD, Demuth H, Wiberg C, Schjødt C, Huang T, Chen J, Jensen S, Petersen J, Thygesen P, Biochemistry (2020) Europe PMC

SASDF42 – Human serum albumin in complex with somapacitan

Human serum albumin
Somapacitan
MWexperimental 113 kDa
MWexpected 111 kDa
VPorod 202 nm3
log I(s) 6.84×100 6.84×10-1 6.84×10-2 6.84×10-3
Human serum albumin Somapacitan small angle scattering data  s, nm-1
ln I(s)
Human serum albumin Somapacitan Guinier plot ln 6.84×100 Rg: 4.1 nm 0 (4.1 nm)-2 s2
(sRg)2I(s)/I(0)
Human serum albumin Somapacitan Kratky plot 1.104 0 √3 sRg
p(r)
Human serum albumin Somapacitan pair distance distribution function Rg: 4.1 nm 0 Dmax: 13.9 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Human serum albumin Somapacitan SASREF model
SAXS data from solutions of HSA in complex with somapacitan in 100 mM MES, 140 mM NaCl, pH 6.5 were collected on a Rigaku BioSAXS-2000 instrument at Novo Nordisk A/S (Maaloev, Denmark) using a Pilatus 100K detector at a wavelength of λ = 0.1542 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 6.30 mg/ml was measured at 25°C. Six successive 3600 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Storage temperature = UNKNOWN. Sample detector distance = UNKNOWN

Human serum albumin (HSA)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   66.5 kDa
 
UniProt   P02768 (25-609)
Sequence   FASTA
 
Somapacitan
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   22.1 kDa
 
UniProt   P01241 (27-217)
Sequence   FASTA