Structure, dynamics and roX2-lncRNA binding of tandem double-stranded RNA binding domains dsRBD1,2 of Drosophila helicase Maleless.

Ankush Jagtap PK, Müller M, Masiewicz P, von Bülow S, Hollmann NM, Chen PC, Simon B, Thomae AW, Becker PB, Hennig J, Nucleic Acids Res 47(8):4319-4333 (2019) Europe PMC

SASDF62 – Mixture of the dsRBD1 and dsRBD2 domains of Drosophila helicase dosage compensation regulator, MLE, and the roX2 RNA stem-loop 7 18mer-fragment, at 1.0 mg/ml

Dosage compensation regulator
roX2 stem-loop 7, 18-mer fragment
MWexperimental 7 kDa
MWexpected 40 kDa
VPorod 25 nm3
log I(s) 4.07×101 4.07×100 4.07×10-1 4.07×10-2
Dosage compensation regulator roX2 stem-loop 7, 18-mer fragment small angle scattering data  s, nm-1
ln I(s)
Dosage compensation regulator roX2 stem-loop 7, 18-mer fragment Guinier plot ln 4.07×101 Rg: 3.1 nm 0 (3.1 nm)-2 s2
(sRg)2I(s)/I(0)
Dosage compensation regulator roX2 stem-loop 7, 18-mer fragment Kratky plot 1.104 0 3 sRg
p(r)
Dosage compensation regulator roX2 stem-loop 7, 18-mer fragment pair distance distribution function Rg: 3.3 nm 0 Dmax: 13.3 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of the dsRBD1 and dsRBD2 domains of Drosophila helicase dosage compensation regulator, MLE, and the roX2 RNA stem-loop 7 18mer-fragment in 20 mM NaPO4, 200 mM NaCl, 1 mM DTT, pH 6.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.09919 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 20°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Dosage compensation regulator (MLE)
Mol. type   Protein
Organism   Drosophila melanogaster
Olig. state   Monomer
Mon. MW   28.5 kDa
 
UniProt   P24785 (1-257)
Sequence   FASTA
 
roX2 stem-loop 7, 18-mer fragment (SL7-18mer)
Mol. type   RNA
Organism   synthetic construct
Olig. state   Monomer
Mon. MW   11.6 kDa
Sequence   FASTA