Structural Insights Into the Transcriptional Regulation of HigBA Toxin–Antitoxin System by Antitoxin HigA in Pseudomonas aeruginosa

Liu Y, Gao Z, Liu G, Geng Z, Dong Y, Zhang H, Frontiers in Microbiology 10 (2020) DOI

SASDF85 – Pseudomonas aeruginosa antitoxin HigA: apo PaHigA

Uncharacterized protein
MWI(0) 22 kDa
MWexpected 22 kDa
VPorod 23 nm3
log I(s) 1.87×101 1.87×100 1.87×10-1 1.87×10-2
Uncharacterized protein small angle scattering data  s, nm-1
ln I(s)
Uncharacterized protein Guinier plot ln 1.88×101 Rg: 2.0 nm 0 (2.0 nm)-2 s2
Uncharacterized protein Kratky plot 1.104 0 3 sRg
Uncharacterized protein pair distance distribution function Rg: 2.0 nm 0 Dmax: 6.6 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Uncharacterized protein PDB (PROTEIN DATA BANK) model

log I(s)
 s, nm-1
Uncharacterized protein DAMMIF model

Synchrotron SAXS data from solutions of apo PaHigA in 20 mM Tris, 300 mM NaCl, 5% (v/v) glycerol, 1 mM PMSF, pH 8 were collected on the BL19U2 beam line at the Shanghai Synchrotron Radiation Facility (SSRF; Shanghai, China) using a Pilatus 1M detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.103 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1 and 5 mg/ml were measured at 23°C. 20 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Uncharacterized protein
Mol. type   Protein
Organism   Pseudomonas aeruginosa
Olig. state   Dimer
Mon. MW   11.2 kDa
UniProt   Q9HVC1
Sequence   FASTA