Structural Insights Into the Transcriptional Regulation of HigBA Toxin–Antitoxin System by Antitoxin HigA in Pseudomonas aeruginosa

Liu Y, Gao Z, Liu G, Geng Z, Dong Y, Zhang H, Frontiers in Microbiology 10 (2020) DOI

SASDF95 – Pseudomonas aeruginosa antitoxin HigA bound to duplex DNA: PaHigA-DNA complex

Uncharacterized protein
DNA Duplex
MWI(0) 74 kDa
MWexpected 43 kDa
VPorod 81 nm3
log I(s) 8.03×101 8.03×100 8.03×10-1 8.03×10-2
Uncharacterized protein DNA Duplex small angle scattering data  s, nm-1
ln I(s)
Uncharacterized protein DNA Duplex Guinier plot ln 8.04×101 Rg: 2.9 nm 0 (2.9 nm)-2 s2
(sRg)2I(s)/I(0)
Uncharacterized protein DNA Duplex Kratky plot 1.104 0 √3 sRg
p(r)
Uncharacterized protein DNA Duplex pair distance distribution function Rg: 3.0 nm 0 Dmax: 9.8 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Uncharacterized protein DNA Duplex PDB (PROTEIN DATA BANK) model
log I(s)
 s, nm-1
Uncharacterized protein DNA Duplex DAMMIF model
Synchrotron SAXS data from solutions of the PaHigA-DNA complex in 20 mM Tris, 300 mM NaCl, 5% (v/v) glycerol, 1 mM PMSF, pH 8 were collected on the BL19U2 beam line at the Shanghai Synchrotron Radiation Facility (SSRF; Shanghai, China) using a Pilatus 1M detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.103 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1 and 5 mg/ml were measured at 23°C. 20 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Uncharacterized protein
Mol. type   Protein
Organism   Pseudomonas aeruginosa
Olig. state   Dimer
Mon. MW   11.2 kDa
 
UniProt   Q9HVC1
Sequence   FASTA
 
DNA Duplex
Mol. type   DNA
Olig. state   Dimer
Mon. MW   10.0 kDa
Sequence   FASTA
 
PDB ID   6JPI