Synchrotron SAXS data from solutions of phosphorylated Ric-8A (amino acids 1-452) in 25mM HEPES, 150mM NaCl, pH 8 were collected using size-exclusion chromatography SAXS (SEC-SAXS) on the BL4-2 beam line at the Stanford Synchrotron Radiation Lightsource (SSRL; Stanford, CA, USA) using a Rayonix MX225-HE detector at a sample-detector distance of 1.7 m and at a wavelength of λ = 0.1127 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed at 25 °C using the following parameters: Column: Superdex 200 Increase 3.2/300 analytical SEC column; Flow rate: 0.05 mL/min; Sample injection concentration: 1.0 mg/mL; Injection volume: 100 μL. The data were collected through the SEC peak of the sample as a series of 25 x 1 second exposures. Each unsubtracted data frame was normalised to the intensity of the transmitted beam and radially averaged and the scattering of an appropriate solvent-blank was subtracted. The resulting subtracted frames were scaled and averaged to generate the final SAXS profile displayed in this entry.
s, nm-1
