Arabidopsis immunity regulator EDS1 in a PAD4/SAG101-unbound form is a monomer with an inherently inactive conformation.

Voss M, Toelzer C, Bhandari DD, Parker JE, Niefind K, J Struct Biol :107390 (2019) Europe PMC

SASDFC5 – Enhanced disease susceptibility 1 (EDS1)

Enhanced disease susceptibility
MWexperimental 72 kDa
MWexpected 72 kDa
VPorod 92 nm3
log I(s) 1.51×102 1.51×101 1.51×100 1.51×10-1
Enhanced disease susceptibility small angle scattering data  s, nm-1
ln I(s)
Enhanced disease susceptibility Guinier plot ln 1.52×102 Rg: 3.1 nm 0 (3.1 nm)-2 s2
Enhanced disease susceptibility Kratky plot 1.104 0 3 sRg
Enhanced disease susceptibility pair distance distribution function Rg: 3.1 nm 0 Dmax: 10.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Enhanced disease susceptibility DAMMIN model

Synchrotron SAXS data from solutions of enhanced disease susceptibility 1 (EDS1) in 50 mM NaCl, 50 mM HEPES, 1% glyercol, 1 mM DTT, pH 8 were collected using size-exclusion chromatography SAXS (SEC-SAXS) on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed at 4 °C using the following parameters: Column: Superdex75 10/300GL; Flow rate: 0.4 mL/min; Sample injection concentration: 5 mg/mL; Injection volume: 500 μL. 1495 successive 2 second frames were collected through the SEC-elution and processed using CHROMIXS. Each unsubtracted data frame was normalised to the intensity of the transmitted beam and radially averaged and the scattering of an appropriate solvent-blank was subtracted from the sample frames. The resulting subtracted frames were scaled and averaged to generate the final SAXS profile displayed in this entry.

The data were re-binned to calculate the p(r) profile and corresponding model fit.

Enhanced disease susceptibility (EDS1)
Mol. type   Protein
Organism   Arabidopsis thaliana
Olig. state   Monomer
Mon. MW   71.7 kDa
UniProt   Q9SU72
Sequence   FASTA