Conformationally constrained mutant of human matrix metalloproteinase-1

Rob Holland.

SASDFM4 – Matrix metalloproteinase-1 (MMP-1)

Matrix metalloproteinase-1 (Interstitial collagenase)
MWexperimental 32 kDa
MWexpected 43 kDa
VPorod 50 nm3
log I(s) 1.34×10-3 1.34×10-4 1.34×10-5 1.34×10-6
Matrix metalloproteinase-1 (Interstitial collagenase) small angle scattering data  s, nm-1
ln I(s)
Matrix metalloproteinase-1 (Interstitial collagenase) Guinier plot ln 1.34×10-3 Rg: 2.6 nm 0 (2.6 nm)-2 s2
(sRg)2I(s)/I(0)
Matrix metalloproteinase-1 (Interstitial collagenase) Kratky plot 1.104 0 3 sRg
p(r)
Matrix metalloproteinase-1 (Interstitial collagenase) pair distance distribution function Rg: 2.6 nm 0 Dmax: 8.3 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of matrix metalloproteinase-1 (MMP-1) in 50 mM Tris-HCl, 150 mM NaCl, 10 mM CaCl2, pH 7.4 were collected using size-exclusion chromatography SAXS (SEC-SAXS) on the B21 beam line at the Diamond Light Source (Oxfordshire, UK) using a Pilatus 2M detector at a sample-detector distance of 3.9 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed at 25 °C using the following parameters: Shodex Kw-403 analytical SEC column; Flow rate: 0.2 mL/min; Sample injection concentration: 8 mg/mL; Injection volume: 45 μL. The data were collected through the SEC peak of the sample as a series of 27 x 3 second exposures. Each unsubtracted data frame was normalised to the intensity of the transmitted beam and radially averaged and the scattering of an appropriate solvent-blank was subtracted. The resulting subtracted frames were scaled and averaged to generate the final SAXS profile displayed in this entry.

SEC column = UNKNOWN. Sample injection volume = UNKNOWN. Flow rate = UNKNOWN

Matrix metalloproteinase-1 (Interstitial collagenase) (MMP-1)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   42.6 kDa
 
UniProt   P03956 (100-469)
Sequence   FASTA