A Tail-Based Mechanism Drives Nucleosome Demethylation by the LSD2/NPAC Multimeric Complex.

Marabelli C, Marrocco B, Pilotto S, Chittori S, Picaud S, Marchese S, Ciossani G, Forneris F, Filippakopoulos P, Schoehn G, Rhodes D, Subramaniam S, Mattevi A, Cell Rep 27(2):387-399.e7 (2019) Europe PMC

SASDFW3 – Cytokine-like nuclear factor dehydrogenase domain, NPAC DH, plus native linker (NPAC delta-205)

NPAC linker+DH (delta-205)
MWexperimental 160 kDa
MWexpected 150 kDa
VPorod 240 nm3
log I(s) 2.41×101 2.41×100 2.41×10-1 2.41×10-2
NPAC linker+DH (delta-205) small angle scattering data  s, nm-1
ln I(s)
NPAC linker+DH (delta-205) Guinier plot ln 2.41×101 Rg: 4.0 nm 0 (4.0 nm)-2 s2
NPAC linker+DH (delta-205) Kratky plot 1.104 0 3 sRg
NPAC linker+DH (delta-205) pair distance distribution function Rg: 4.0 nm 0 Dmax: 11.4 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of truncated cytokine-like nuclear factor (NPAC delta-205) in 15 mM HEPES, 200 mM NaCl, pH 7.3 were collected using size-exclusion chromatography SAXS (SEC-SAXS) on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.1 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed at 20 °C using the following parameters: Column: Wyatt WTC-030N5; Flow rate: 0.25 mL/min; Sample injection concentration: 2 mg/mL; Injection volume: 50 μL. The data were collected through the SEC peak of the sample as a series of 222 x 1 second exposures. Each unsubtracted data frame was normalised to the intensity of the transmitted beam and radially averaged and the scattering of an appropriate solvent-blank was subtracted. The resulting subtracted frames were scaled and averaged to generate the final SAXS profile displayed in this entry.

The protein construct encompasses the dehydrogenase domain plus an additional upstream native-sequence amino acid linker (compare to the isolated dehydrogenase domain, SASDFV3, NPAC delta-261).

NPAC linker+DH (delta-205) (NPAC delta205)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Tetramer
Mon. MW   37.4 kDa
UniProt   Q49A26 (205-553)
Sequence   FASTA