Apolipoprotein E project

Lucas Kraft.

SASDFY8 – Apolipoprotein E2 (ApoE2) bound to 110 µg/mL heparin

Apolipoprotein E2
MWexperimental 259 kDa
MWexpected 154 kDa
VPorod 666 nm3
log I(s) 6.59×10-1 6.59×10-2 6.59×10-3 6.59×10-4
Apolipoprotein E2 Heparin small angle scattering data  s, nm-1
ln I(s)
Apolipoprotein E2 Heparin Guinier plot ln 6.59×10-1 Rg: 7.1 nm 0 (7.1 nm)-2 s2
Apolipoprotein E2 Heparin Kratky plot 1.104 0 3 sRg
Apolipoprotein E2 Heparin pair distance distribution function Rg: 7.2 nm 0 Dmax: 24 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of ApoE2 bound to 110 µg/mL heparin in 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH 8 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Pilatus 2M detector at a sample-detector distance of 4.0 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 4.00 mg/ml was measured at 20°C. 28 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

ApoE2 + 110 µg/mL heparin. Molecular weight estimates are inaccurate due to heparin heterogeneity.

Apolipoprotein E2 (ApoE2)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Tetramer
Mon. MW   34.6 kDa
UniProt   P02649 (19-317)
Sequence   FASTA
Mol. type   Other
Olig. state   Monomer
Mon. MW   15 kDa
Chemical formula