Structural basis for distinct roles of SMAD2 and SMAD3 in FOXH1 pioneer-directed TGF-β signaling.

Aragón E, Wang Q, Zou Y, Morgani SM, Ruiz L, Kaczmarska Z, Su J, Torner C, Tian L, Hu J, Shu W, Agrawal S, Gomes T, Márquez JA, Hadjantonakis AK, Macias MJ, Massagué J, Genes Dev 33(21-22):1506-1524 (2019) Europe PMC

SASDG35 – Mothers against decapentaplegic homolog 2, S2MH1E3

Mothers against decapentaplegic homolog 2
MWexperimental 20 kDa
MWexpected 19 kDa
VPorod 35 nm3
log I(s) 2.07×101 2.07×100 2.07×10-1 2.07×10-2
Mothers against decapentaplegic homolog 2 small angle scattering data  s, nm-1
ln I(s)
Mothers against decapentaplegic homolog 2 Guinier plot ln 2.08×101 Rg: 1.9 nm 0 (1.9 nm)-2 s2
(sRg)2I(s)/I(0)
Mothers against decapentaplegic homolog 2 Kratky plot 1.104 0 √3 sRg
p(r)
Mothers against decapentaplegic homolog 2 pair distance distribution function Rg: 1.9 nm 0 Dmax: 7.4 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of S2MH1E3 in 20 mM Tris 150 mM NaCl, pH 7.2 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1 and 5 mg/ml were measured at 10°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Sample detector distance = UNKNOWN. X-ray Exposure time = UNKNOWN. Number of frames = UNKNOWN

Mothers against decapentaplegic homolog 2
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   19.0 kDa
 
UniProt   Q15796 (10-174)
Sequence   FASTA