Mannose receptor (CD206) activation in tumor-associated macrophages enhances adaptive and innate antitumor immune responses.

Jaynes JM, Sable R, Ronzetti M, Bautista W, Knotts Z, Abisoye-Ogunniyan A, Li D, Calvo R, Dashnyam M, Singh A, Guerin T, White J, Ravichandran S, Kumar P, Talsania K, Chen V, Ghebremedhin A, Karanam B, Bin Salam A, Amin R, Odzorig T, Aiken T, Nguyen V, Bian Y, Zarif JC, de Groot AE, Mehta M, Fan L, Hu X, Simeonov A, Pate N, Abu-Asab M, Ferrer M, Southall N, Ock CY, Zhao Y, Lopez H, Kozlov S, de Val N, Yates CC, Baljinnyam B, Marugan J, Rudloff U, Sci Transl Med 12(530) (2020) Europe PMC

SASDG54 – Human macrophage mannose receptor 1 protein

Macrophage mannose receptor 1
MWexperimental 345 kDa
MWexpected 315 kDa
VPorod 584 nm3
log I(s) 5.30×10-1 5.30×10-2 5.30×10-3 5.30×10-4
Macrophage mannose receptor 1 small angle scattering data  s, nm-1
ln I(s)
Macrophage mannose receptor 1 Guinier plot ln 5.30×10-1 Rg: 7.9 nm 0 (7.9 nm)-2 s2
Macrophage mannose receptor 1 Kratky plot 1.104 0 3 sRg
Macrophage mannose receptor 1 pair distance distribution function Rg: 8.2 nm 0 Dmax: 30.1 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Macrophage mannose receptor 1 ITASSER model

Synchrotron SAXS data from solutions of human macrophage mannose receptor 1 in 50 mM HEPES, 100 mM NaCl, 1 mM DTT, pH 7 were collected on the 12ID-B SAXS/WAXS beam line at the Advanced Photon Source (APS), Argonne National Laboratory (Lemont, IL, USA) using a Pilatus 2M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.093 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.9 and 1.8 mg/ml were measured at 20°C. 30 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.

Macrophage mannose receptor 1 (MRC1 or CD206)
Mol. type   Protein
Organism   Mouse myeloma cell line
Olig. state   Dimer
Mon. MW   157.5 kDa
UniProt   P22897 (19-1388+6-his)
Sequence   FASTA